0726. The effects of carnitine on renal tissue damage and metabolic alteration among the rat intraabdominal sepsis model

Methods 21 male rats randomly divided into 3 groups: sham operated (SO; n=7), sepsis (S; n=7) and sepsis + carnitine (S+C; n=7). In SO group only laparatomy was performed. In S and S+C groups caecal ligation and puncture was performed. At the postoperative 6, 30 and 54 hours 1ml saline was administered intraperitoneally to both PO and S groups. Similarly 100mg/kg carnitine was administered intraperitoneally to S+C group at the postoperative 6, 30 and 54 hours. After gathering blood and renal tissue samples, all rats were sacrificed at 60 hours postoperatively. Tissue malondialdehyde (MDA) levels were measured for oxidative stress and inflammation. Serum cytokines (TNF-a, IL-1, IL-6 and IL-10), neutrophil gelatinase-associated lipocalin (NGAL) and anti-caspase 3 were measured for inflammation markers, renal tissue damage and renal apoptosis, respectively. Results In histopathological examination, S+C group has achieved better results in mononuclear cell infiltration score than the S group. Also, the increase in TNF-a, IL-1, IL-6, MDA, NGAL and anti-caspase 3 levels in S+C group were found to be lower than those of S group.